3P299 Observation of the fluorescence change of green fluorescence protein under mechanical perturbation with an AFM probe
نویسندگان
چکیده
منابع مشابه
Fluorescence polarization of green fluorescence protein.
We report here the striking anisotropy of fluorescence exhibited by crystals of native green fluorescence protein (GFP). The crystals were generated by water dialysis of highly purified GFP obtained from the jellyfish Aequorea. We find that the fluorescence becomes six times brighter when the excitation, or emission, beam is polarized parallel (compared with perpendicular) to the crystal long a...
متن کاملStudy of the flexural sensitivity and resonant frequency of an inclined AFM cantilever with sidewall probe
The resonant frequency and sensitivity of an atomic force microscope (AFM) cantilever with assembled cantilever probe (ACP) have been analyzed and a closed-form expression for the sensitivity of vibration modes has been obtained. The proposed ACP comprises an inclined cantilever and extension, and a tip located at the free end of the extension, which makes the AFM capable of topography at sidew...
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چکیده ندارد.
15 صفحه اولStudy of the size-dependant vibration behavior of an AFM microcantilever with a sidewall probe
In this paper, the resonant frequency and sensitivity of an atomic force microscope (AFM) with an assembled cantilever probe (ACP) are analyzed utilizing the modified couple stress theory. The proposed ACP comprises a horizontal microcantilever, an extension and a tip located at the free end of the extension, which make AFM capable of scanning the sample sidewall. First, the governing different...
متن کاملStudy of the flexural sensitivity and resonant frequency of an inclined AFM cantilever with sidewall probe
The resonant frequency and sensitivity of an atomic force microscope (AFM) cantilever with assembled cantilever probe (ACP) have been analyzed and a closed-form expression for the sensitivity of vibration modes has been obtained. The proposed ACP comprises an inclined cantilever and extension, and a tip located at the free end of the extension, which makes the AFM capable of topography at sidew...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2004
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.44.s264_3